Microvolume dynamic light scattering and simultaneous video microscopic observation for screening of protein crystal growth

Thomas Wessel; Jaro Ricka

doi:10.1117/12.301121

1 January 1998 Microvolume dynamic light scattering and simultaneous video microscopic observation for screening of protein crystal growth

Thomas Wessel, Jaro Ricka

Author Affiliations +

Proceedings Volume 3199, Biomedical Systems and Technologies II; (1998) https://doi.org/10.1117/12.301121
Event: BiOS Europe '97, 1997, San Remo, Italy

Abstract

Dynamic light scattering (DLS), video microscopic and ultra- microscopic observation were simultaneously employed for screening of protein crystal growth in the vapor diffusion regime. The setup, consisting of a modified microscope equipped with a laser sheet (darkfield) illumination for ultra-microscopy, allows the visualization of clusters in the nanometer range as well as of macroscopic crystals parallel with the DLS measurements. The results of microscopic and ultra-microscopic observations, the DLS autocorrelation functions and the resulting relaxation time distributions for the standard protein lysozyme are presented. The special conditions for dynamic light scattering measurements in the pre and post crystallization phase with samples situated in small droplets and covered by a standard crystallization plate are discussed.

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Thomas Wessel and Jaro Ricka "Microvolume dynamic light scattering and simultaneous video microscopic observation for screening of protein crystal growth", Proc. SPIE 3199, Biomedical Systems and Technologies II, (1 January 1998); https://doi.org/10.1117/12.301121

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