Two-dimensional fluorescence lifetime imaging for in-vitro and in-vivo application

Paul M. W. French; Mark J. Dayel; Keith Dowling; Sam C. W. Hyde; M. John Lever; P. Vourdas; Anthony K. L. Dymoke-Bradshaw; Jonathan D. Hares

doi:10.1117/12.305374

16 April 1998 Two-dimensional fluorescence lifetime imaging for in-vitro and in-vivo application

Paul M. W. French, Mark J. Dayel, Keith Dowling, Sam C. W. Hyde, M. John Lever, P. Vourdas, Anthony K. L. Dymoke-Bradshaw, Jonathan D. Hares

Author Affiliations +

Proceedings Volume 3250, Optical Biopsy II; (1998) https://doi.org/10.1117/12.305374
Event: BiOS '98 International Biomedical Optics Symposium, 1998, San Jose, CA, United States

Abstract

We report the development of a fluorescence lifetime imaging (FLIM) system based on a time-gated image intensifier and solid-state laser amplifier with a system response of < 100 ps. We have sued this system to image lifetimes as short as 100 ps and to image changes in the environment of a fluorescent phantom, causing lifetime differences less than 10 ps. The versatility of this FLIM system has been demonstrated by measuring both the temporal and spectral profiles of multiple fluorescent samples in a single acquisition. Fluorescence lifetime imaging of tissue constituents has also been carried out and first results suggest that this technique can provide a means of distinguishing between different tissue constituents.

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Paul M. W. French, Mark J. Dayel, Keith Dowling, Sam C. W. Hyde, M. John Lever, P. Vourdas, Anthony K. L. Dymoke-Bradshaw, and Jonathan D. Hares "Two-dimensional fluorescence lifetime imaging for in-vitro and in-vivo application", Proc. SPIE 3250, Optical Biopsy II, (16 April 1998); https://doi.org/10.1117/12.305374

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