Development of flow-cytometry-based miniature chemical fluid analysis system using fluorescent microbeads

Ming-Chieh Huang; Martin A. Afromowitz; Ger van den Engh; Bernhard H. Weigl; Paul Yager

doi:10.1117/12.307061

1 May 1998 Development of flow-cytometry-based miniature chemical fluid analysis system using fluorescent microbeads

Ming-Chieh Huang, Martin A. Afromowitz, Ger van den Engh, Bernhard H. Weigl, Paul Yager

Author Affiliations +

Proceedings Volume 3256, Advances in Optical Biophysics; (1998) https://doi.org/10.1117/12.307061
Event: BiOS '98 International Biomedical Optics Symposium, 1998, San Jose, CA, United States

Abstract

We present a demonstration of an optical microfluidic system for rapid and simultaneous measurement of several fluid analytes in a microscale volume that is based on fluorescent indicators immobilized on polymer microbeads. The fluorescence properties of such 'reporter beads' can be a function of the concentration of an analyte, and can be monitored in a silicon microfabricated flow channel. The sizes of microbeads can be determined by the scattering signal, so this system can be used to analyze several analytes at the same time by using beads of different sizes that are sensitive to different analytes. The fluorescent indicator Carboxy-SNAFL 1 was immobilized on amino-functionalized polystyrene beads of 5 micrometer diameter. The intensities of two fluorescent peaks (at 560 nm and at 620 nm) were measured and their ratio was dependent on the pH value of the analyte. A 488 nm air-cooled argon laser was used to excite the fluorescent beads flowing through a microfabricated V-groove channel. Two miniature photomultiplier modules and interference filters were used to simultaneously measure both fluorescence intensities.

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Ming-Chieh Huang, Martin A. Afromowitz, Ger van den Engh, Bernhard H. Weigl, and Paul Yager "Development of flow-cytometry-based miniature chemical fluid analysis system using fluorescent microbeads", Proc. SPIE 3256, Advances in Optical Biophysics, (1 May 1998); https://doi.org/10.1117/12.307061

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