Determination of cell culture medium components with overlapping near-IR absorbance peaks

Michael J. McShane; Gerard L. Cote

doi:10.1117/12.306108

24 April 1998 Determination of cell culture medium components with overlapping near-IR absorbance peaks

Michael J. McShane, Gerard L. Cote

Proceedings Volume 3257, Infrared Spectroscopy: New Tool in Medicine; (1998) https://doi.org/10.1117/12.306108
Event: BiOS '98 International Biomedical Optics Symposium, 1998, San Jose, CA, United States

Abstract

A noninvasive method to measure levels of important tissue culture nutrients and metabolic by-products would provide the potential for computer control of the growth process, decreasing the amount of human attention necessary while improving efficiency and repeatability of experiments by precise maintenance of important parameters without contaminating the culture environment. Our approach to meeting this need is the development of optical sensor that extract chemical information based on the absorbance of near-IR radiation. Near-IR spectroscopy of the 2.0-2.5 micrometers region was used to collect absorbance data of aqueous mixtures containing glucose, lactate, ammonia, glutamate, and glutamine, as well as cell culture media samples from a three-day growth period. Multivariate calibration was used with these data to prove that complicated spectra can yield reasonable prediction errors. Calibration models using different combinations of spectra from aqueous mixtures and culture media were built and evaluated for glucose, ammonia, and lactate. Prediction errors of 2 percent, 8 percent, and 15 percent were obtained for glucose, lactate, and ammonia, respectively.

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Michael J. McShane and Gerard L. Cote "Determination of cell culture medium components with overlapping near-IR absorbance peaks", Proc. SPIE 3257, Infrared Spectroscopy: New Tool in Medicine, (24 April 1998); https://doi.org/10.1117/12.306108

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KEYWORDS

Calibration

Glucose

Data modeling

Absorbance

Statistical modeling

Error analysis

Near infrared spectroscopy

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