Rapid identification of the multidrug resistance in the human leukemic cells by near-infrared Fourier transform Raman microspectroscopy

Abdelilah Beljebbar; Hamid Morjani; Ganesh D. Sockalingum; Michel Manfait

doi:10.1117/12.306106

24 April 1998 Rapid identification of the multidrug resistance in the human leukemic cells by near-infrared Fourier transform Raman microspectroscopy

Abdelilah Beljebbar, Hamid Morjani, Ganesh D. Sockalingum, Michel Manfait

Author Affiliations +

Proceedings Volume 3257, Infrared Spectroscopy: New Tool in Medicine; (1998) https://doi.org/10.1117/12.306106
Event: BiOS '98 International Biomedical Optics Symposium, 1998, San Jose, CA, United States

Abstract

In this work, we have studied the cancer cell lines sharing a common feature: the multi-drug resistance where P- glycoprotein is responsible for the active efflux of the drug out of the cell. For this, we have used two types of cells, MDR-human leukemic K562 cells and non-MDR acute promyelocytic leukemic HL60 cells. The comparison between normalized micro FT-Raman spectra of resistant and sensitive K652 cells shows a decrease in the intensity of the amide I and III bands and a down shift of the amide I band. On the other hand, control experiments with HL60 cells do not show any remarkable changes. Analysis of micro-FT-Raman spectra by resolution enhancement methods and by chemometrics tools reveal further information concerning the conformational changes of the cell constituents involved in the expression of the MDR-phenotype.

Citation Download Citation

Abdelilah Beljebbar, Hamid Morjani, Ganesh D. Sockalingum, and Michel Manfait "Rapid identification of the multidrug resistance in the human leukemic cells by near-infrared Fourier transform Raman microspectroscopy", Proc. SPIE 3257, Infrared Spectroscopy: New Tool in Medicine, (24 April 1998); https://doi.org/10.1117/12.306106

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