Light scattering from metal sol labels on high-density DNA probe arrays

Mark O. Trulson; Ian D. Walton; Audrey D. Suseno; Hajime Matsuzaki; David Stern

doi:10.1117/12.307333

10 April 1998 Light scattering from metal sol labels on high-density DNA probe arrays

Mark O. Trulson, Ian D. Walton, Audrey D. Suseno, Hajime Matsuzaki, David Stern

Proceedings Volume 3259, Systems and Technologies for Clinical Diagnostics and Drug Discovery; (1998) https://doi.org/10.1117/12.307333
Event: BiOS '98 International Biomedical Optics Symposium, 1998, San Jose, CA, United States

Abstract

We have been exploring the use of light scattering as a means to detect the binding of nucleic acids to high density DNA probe arrays. Initial work has concentrated on the use of 100 nanometer gold particles conjugated to monoclonal antibodies. A probe array scanner that utilizes an arc lamp source and a `photocopier grade' linear CCD detector has been developed. The optical configuration of the scanner maximizes dynamic range and minimizes optical backgrounds. Initial development of light scattering detection for the p53 cancer gene application shows that functional performance may be obtained that is essentially equivalent to existing fluorescence detection methodology.

Citation Download Citation

Mark O. Trulson, Ian D. Walton, Audrey D. Suseno, Hajime Matsuzaki, and David Stern "Light scattering from metal sol labels on high-density DNA probe arrays", Proc. SPIE 3259, Systems and Technologies for Clinical Diagnostics and Drug Discovery, (10 April 1998); https://doi.org/10.1117/12.307333

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