Ultrafast lasers and biological applications: from two-photon to molecular relaxation imaging

Arjan H. Buist; G. J. Brakenhoff; Michiel Mueller; Theodore B. Norris; Jeffrey A. Squier; Chris J. Bardeen; Vladislav V. Yakovlev; Kent R. Wilson

doi:10.1117/12.312335

8 July 1998 Ultrafast lasers and biological applications: from two-photon to molecular relaxation imaging

Arjan H. Buist, G. J. Brakenhoff, Michiel Mueller, Theodore B. Norris, Jeffrey A. Squier, Chris J. Bardeen, Vladislav V. Yakovlev, Kent R. Wilson

Author Affiliations +

Proceedings Volume 3269, Commercial Applications of Ultrafast Lasers; (1998) https://doi.org/10.1117/12.312335
Event: Optoelectronics and High-Power Lasers and Applications, 1998, San Jose, CA, United States

Abstract

This contribution discusses some biological applications of ultrashort laser pulses. Some examples are given of recently developed techniques that exploit the special features offered by ultrashort laser pulses: real time two-photon microscopy with multipoint excitation, fluorescence lifetime measurement by double pulse saturation excitation and pH-sensing by multiphoton quantum control.

Citation Download Citation

Arjan H. Buist, G. J. Brakenhoff, Michiel Mueller, Theodore B. Norris, Jeffrey A. Squier, Chris J. Bardeen, Vladislav V. Yakovlev, and Kent R. Wilson "Ultrafast lasers and biological applications: from two-photon to molecular relaxation imaging", Proc. SPIE 3269, Commercial Applications of Ultrafast Lasers, (8 July 1998); https://doi.org/10.1117/12.312335

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