Sensitive on-column absorbance detection of native molecules

Sandrine Berniolles; Hongjing Kan; Lu Dai; James E. Knittle; William M. Tong

doi:10.1117/12.308380

15 May 1998 Sensitive on-column absorbance detection of native molecules

Sandrine Berniolles, Hongjing Kan, Lu Dai, James E. Knittle, William M. Tong

Proceedings Volume 3270, Methods for Ultrasensitive Detection; (1998) https://doi.org/10.1117/12.308380
Event: Optoelectronics and High-Power Lasers and Applications, 1998, San Jose, CA, United States

Abstract

Laser wave mixing is presented as a sensitive detection method for absorbance measurements in flowing liquid analytes. Wave mixing is an unusually sensitive multi-photon nonlinear optical method since the analytical signal is generated as a coherent laser beam. Since the bright signal is visible to the naked eye, optical alignment is convenient. For liquid analytes in continuously flowing cells, we have demonstrated excellent detection sensitivity levels using various wave-mixing optical configurations and laser sources. Since it is an optical absorption method, laser wave-mixing detection offers excellent detection sensitivity for both fluorescing and non-fluorescing analytes. Hence, one does not have to label non-fluorescing analytes with tags in order to obtain good detection sensitivity in wave-mixing detection methods. Sensitivity detection of analytes in their native form offers many obvious advantages especially when interfaced to popular capillary separation methods. Since the analyte laser probe volume is very small, wave-mixing detection is suitable for on-column detection in various capillary electrophoresis or micro liquid chromatography systems.

Citation Download Citation

Sandrine Berniolles, Hongjing Kan, Lu Dai, James E. Knittle, and William M. Tong "Sensitive on-column absorbance detection of native molecules", Proc. SPIE 3270, Methods for Ultrasensitive Detection, (15 May 1998); https://doi.org/10.1117/12.308380

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