Development of a versatile organophosphorous-hydrolase-based assay for organophosphate pesticides

Kim R. Rogers; Yi Wang; Ashok Mulchandani; P. Mulchandani; Wilfred Chen

doi:10.1117/12.339023

10 February 1999 Development of a versatile organophosphorous-hydrolase-based assay for organophosphate pesticides

Kim R. Rogers, Yi Wang, Ashok Mulchandani, P. Mulchandani, Wilfred Chen

Proceedings Volume 3534, Environmental Monitoring and Remediation Technologies; (1999) https://doi.org/10.1117/12.339023
Event: Photonics East (ISAM, VVDC, IEMB), 1998, Boston, MA, United States

Abstract

We report a rapid and versatile organophosphorus hydrolase (OPH)-based method for measurement of organophosphate pesticides. This assay is based on a substrate-dependant change in pH near the active site of the enzyme. The pH change is monitored using fluorescein isothiocyanate (FITC) which is covalently immobilized to the enzyme. This method employs FITC-labeled enzyme adsorbed to polymethylmethacrylate beads. Analytes were measured using a microbead fluorescence analyzer. The dynamic concentration range for the assay extends from 25 (mu) M to 400 (mu) M for paraoxon with a detection limit of 8 (mu) M. This assay compared favorably to an HPLC method for monitoring the concentration of coumaphos in bioremediation filtrate samples.

Citation Download Citation

Kim R. Rogers, Yi Wang, Ashok Mulchandani, P. Mulchandani, and Wilfred Chen "Development of a versatile organophosphorous-hydrolase-based assay for organophosphate pesticides", Proc. SPIE 3534, Environmental Monitoring and Remediation Technologies, (10 February 1999); https://doi.org/10.1117/12.339023

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