Microscope-based single photon imaging for studying the functional dynamics of living cells using luminesent markers

Eric Karplus; Anastasia Kanellopoulos

doi:10.1117/12.351034

2 July 1999 Microscope-based single photon imaging for studying the functional dynamics of living cells using luminesent markers

Eric Karplus, Anastasia Kanellopoulos

Author Affiliations +

Proceedings Volume 3600, Biomedical Imaging: Reporters, Dyes, and Instrumentation; (1999) https://doi.org/10.1117/12.351034
Event: BiOS '99 International Biomedical Optics Symposium, 1999, San Jose, CA, United States

Abstract

A microscope-based imaging system has been developed that records single photon emissions from luminescent samples. The time and position of each detected photon is recorded so that integration times for image reconstruction can be selected and adjusted during data analysis. The system performs automated acquisition of brightfield and epifluorescence images that are correlated with the photon images. The features of this system will be demonstrated in the context of studying the dynamics of living cells using luminescent markers such as luciferase and aequorin, which can be introduced by microinjection or transfection. The value of using this approach to study the effects of pharmacological agents on gene expression in living cells will be presented.

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Eric Karplus and Anastasia Kanellopoulos "Microscope-based single photon imaging for studying the functional dynamics of living cells using luminesent markers", Proc. SPIE 3600, Biomedical Imaging: Reporters, Dyes, and Instrumentation, (2 July 1999); https://doi.org/10.1117/12.351034

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