Characterizing high-quality microscope objectives: a new approach

Rimas Juskaitis; Mark A. A. Neil; Tony Wilson

doi:10.1117/12.347584

6 May 1999 Characterizing high-quality microscope objectives: a new approach

Rimas Juskaitis, Mark A. A. Neil, Tony Wilson

Proceedings Volume 3605, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing VI; (1999) https://doi.org/10.1117/12.347584
Event: BiOS '99 International Biomedical Optics Symposium, 1999, San Jose, CA, United States

Abstract

We present a novel technique for testing of the high quality microscope objective lenses. The characterization of the lens is achieved by using a point light source approximated by a 40 nm colloidal gold bead scatterer and simultaneously measuring the field distribution in the pupil plane (pupil function) and light intensity in the image plane (point spread function). Aberrations introduced by the lens are then expanded into Zernike polynomials. The proposed technique is particularly suited for measuring apodization and vignetting effects and allows for easy measurements of the off-axis aberrations.

Citation Download Citation

Rimas Juskaitis, Mark A. A. Neil, and Tony Wilson "Characterizing high-quality microscope objectives: a new approach", Proc. SPIE 3605, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing VI, (6 May 1999); https://doi.org/10.1117/12.347584

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