Photodynamic modulation of immune properties of blood cells

Dmitry Lapotko; Tat'yana Romanovskaya; Vladimir P. Zharov

doi:10.1117/12.388076

13 June 2000 Photodynamic modulation of immune properties of blood cells

Dmitry Lapotko, Tat'yana Romanovskaya, Vladimir P. Zharov

Proceedings Volume 3914, Laser-Tissue Interaction XI: Photochemical, Photothermal, and Photomechanical; (2000) https://doi.org/10.1117/12.388076
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States

Abstract

The mechanisms of therapeutic action of laser radiation with and without photosensitizers were studied experimentally at cell level for living leukocytes. Single cell response to action of pathogens and to laser-based therapeutic processes was measured with photothermal (PT) image microscope. We have monitored cell functional properties during phagocytosis of bacteria S.aureus by human leukocytes. Laser therapy was applied in vitro to cell suspense and to the mixture of cells and microbes in three regimes: (1) low-power (30 - 50 mW/cm²) CW-laser radiation at 633 nm and at 670 nm; (2) photoactivated with 670 nm laser radiation Chlorin E6 at 0.2 mg/l (PDT); (3) photoactivated with 670 nm laser radiation aluminum disulfonated phthalocyanine (Photosens) at 0.2 mg/l (PDT). For all three regimes an increase of bactericidal effect was found only when the cells are involved into interaction with microbes. No bactericidal effect in the wide range of drug doses was found during direct application of PDT to bacteria in vitro. The best effect was found when the cells are treated with laser radiation only. Also laser radiation restored cell properties. As a result we suggest that immune stimulation depends more upon physical factors (laser) rather than upon pharmaceutical (photosensitizers).

Citation Download Citation

Dmitry Lapotko, Tat'yana Romanovskaya, and Vladimir P. Zharov "Photodynamic modulation of immune properties of blood cells", Proc. SPIE 3914, Laser-Tissue Interaction XI: Photochemical, Photothermal, and Photomechanical, (13 June 2000); https://doi.org/10.1117/12.388076

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