pH influence on near-infrared spectra of glucose

Christopher B. Lewis; Gerard L. Cote

doi:10.1117/12.387128

31 May 2000 pH influence on near-infrared spectra of glucose

Christopher B. Lewis, Gerard L. Cote

Proceedings Volume 3923, Optical Diagnostics of Biological Fluids V; (2000) https://doi.org/10.1117/12.387128
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States

Abstract

The objective of this study was to determine the effect of pH on near-infrared (NIR) (2.0 - 2.5 micrometers ) spectra of glucose in phosphate buffered saline solution within a tight range of control similar to what is found in many mammalian organisms and cell cultures. It is known that the NIR water spectrum in the wavelength range (2 - 2.5 micrometers ) may be altered by pH, and it has been shown that glucose absorbance spectra do not change with pH when an equal reference pH is available. However, because the glucose absorbance spectra rides on the water spectra, changes in the spectral characteristics did occur. These effects need to be considered, in particular for single beam glucose spectra.

Citation Download Citation

Christopher B. Lewis and Gerard L. Cote "pH influence on near-infrared spectra of glucose", Proc. SPIE 3923, Optical Diagnostics of Biological Fluids V, (31 May 2000); https://doi.org/10.1117/12.387128

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