Multiparametric analysis of cellular and subcellular function using spectral imaging

Ronald M. Lynch; Kevin D. Nullmeyer; Bridget K. Ford; Linda S. Tompkins; Vicki L. Sutherland; Michael R. Descour

doi:10.1117/12.384242

26 April 2000 Multiparametric analysis of cellular and subcellular function using spectral imaging

Ronald M. Lynch, Kevin D. Nullmeyer, Bridget K. Ford, Linda S. Tompkins, Vicki L. Sutherland, Michael R. Descour

Author Affiliations +

Proceedings Volume 3924, Molecular Imaging: Reporters, Dyes, Markers, and Instrumentation; (2000) https://doi.org/10.1117/12.384242
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States

Abstract

Development of fluorescent probes which sense cellular constituents (metabolites, ions) has revolutionized studies of cell physiology. To take full advantage of these probes for physiological studies, techniques which simultaneously monitor signal from multiple probes with high temporal resolution are required. In addition,the response of unique cells within a population may be heterogeneous making spatial sampling important for specific applications.

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Ronald M. Lynch, Kevin D. Nullmeyer, Bridget K. Ford, Linda S. Tompkins, Vicki L. Sutherland, and Michael R. Descour "Multiparametric analysis of cellular and subcellular function using spectral imaging", Proc. SPIE 3924, Molecular Imaging: Reporters, Dyes, Markers, and Instrumentation, (26 April 2000); https://doi.org/10.1117/12.384242

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