Confocal imaging of biological tissues using second harmonic generation

Beop-Min Kim; Patrick Christian Stoller; Karen M. Reiser; Juergen P. Eichler; Ming Yan; Alexander M. Rubenchik; Luiz Barroca Da Silva

doi:10.1117/12.382051

14 April 2000 Confocal imaging of biological tissues using second harmonic generation

Beop-Min Kim, Patrick Christian Stoller, Karen M. Reiser, Juergen P. Eichler, Ming Yan, Alexander M. Rubenchik, Luiz Barroca Da Silva

Author Affiliations +

Proceedings Volume 3927, Optical Pulse and Beam Propagation II; (2000) https://doi.org/10.1117/12.382051
Event: Symposium on High-Power Lasers and Applications, 2000, San Jose, CA, United States

Abstract

A confocal microscopy imaging system was devised to selectively detect second harmonic signals generated by biological tissues. Several types of biological tissues were examined using this imaging system, including human teeth, bovine blood vessels, and chicken skin. All these tissues generated strong second harmonic signals. There is considerable evidence that the source of these signals in tissue is collagen. Collagen, the predominant component of most tissues, is known to have second order nonlinear susceptibility. This technique may have diagnostic usefulness in pathophysiological conditions characterized by changes in collagen structure including malignant transformation of nevi, progression of diabetic complications, and abnormalities in wound healing.

Citation Download Citation

Beop-Min Kim, Patrick Christian Stoller, Karen M. Reiser, Juergen P. Eichler, Ming Yan, Alexander M. Rubenchik, and Luiz Barroca Da Silva "Confocal imaging of biological tissues using second harmonic generation", Proc. SPIE 3927, Optical Pulse and Beam Propagation II, (14 April 2000); https://doi.org/10.1117/12.382051

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