Detection and identification of animal and food pathogens using time-resolved fluorescence

Peter E. Andreotti; Richard Meyer; Thomas Campbell; Michael T. Goode; Deborah L. Menking; Emily D. Myers; Tom Palenius; Larry H. Stanker

doi:10.1117/12.418741

13 March 2001 Detection and identification of animal and food pathogens using time-resolved fluorescence

Peter E. Andreotti, Richard Meyer, Thomas Campbell, Michael T. Goode, Deborah L. Menking, Emily D. Myers, Tom Palenius, Larry H. Stanker

Author Affiliations +

Proceedings Volume 4206, Photonic Detection and Intervention Technologies for Safe Food; (2001) https://doi.org/10.1117/12.418741
Event: Environmental and Industrial Sensing, 2000, Boston, MA, United States

Abstract

Time-reso1ved fluorescence immunoassays using dissociation enhanced lanthanide fluorescence have been developed for detection and identification of animal and food pathogens including Clostridium botulinum toxins A and B, Staphylococcus enterotoxm B (SEB), Bacillus anthracis, E. coil 0157:H7, Salmonella, Listeria and Campylobacter. Both double antibody sandwich inimunoassays and microwell filterplate assays have been developed using polyclonal, monoclonal and phage display antibodies labeled with fluorescent europium or samarium lanthanide chelates. Multiplexed dual label eiiropium and samarium assays have been performed for Salmonella simultaneously with E. coli 0157:H7, Salmonella and Listeria. Results from different laboratories are presented to demonstrate that these time-resolved fluorescent immunoassays have high sensitivity and specificity with excellent reproducibility.

Citation Download Citation

Peter E. Andreotti, Richard Meyer, Thomas Campbell, Michael T. Goode, Deborah L. Menking, Emily D. Myers, Tom Palenius, and Larry H. Stanker "Detection and identification of animal and food pathogens using time-resolved fluorescence", Proc. SPIE 4206, Photonic Detection and Intervention Technologies for Safe Food, (13 March 2001); https://doi.org/10.1117/12.418741

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