Dynamic light scattering in veterinary medicine: refinement of diagnostic criteria

Stephen Dubin; Stanley Zietz; Karl L. Gabriel D.V.M.; David Gabriel; Michael A. DellaVecchia M.D.; Rafat R. Ansari

doi:10.1117/12.427768

21 May 2001 Dynamic light scattering in veterinary medicine: refinement of diagnostic criteria

Stephen Dubin, Stanley Zietz, Karl L. Gabriel D.V.M., David Gabriel, Michael A. DellaVecchia M.D., Rafat R. Ansari

Proceedings Volume 4244, Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems XI; (2001) https://doi.org/10.1117/12.427768
Event: BiOS 2001 The International Symposium on Biomedical Optics, 2001, San Jose, CA, United States

Abstract

In dynamic light scattering (DLS), the structure or material of interest, suspended in a fluid, is illuminated by a beam of laser light and the scattered light is interpreted in terms of diffusion coefficient, particle size or its distribution. DLS has shown clear promise as a non-invasive, objective and precise diagnostic modality for investigation of lens opacity (cataract) and other medical and toxicological problems. The clinical potential of LDS has been demonstrated in several species both in vivo and in vitro. In many clinical cases, discernment between normal and diseased patients is possible by simple inspection of the particle size distribution. However a more rigorous and sensitive classification scheme is needed, particularly for evaluation of therapy and estimation of tissue injury. The data supplied by DLS investigation is inherently multivariate and its most efficient interpretation requires a multivariate approach which includes the variability among specimens as well as any correlation among the variables (e.g. across the particle size distribution). We present a brief review of DLS methodology, illustrative data and our efforts toward a diagnostic classification scheme. In particular we will describe application of the Mahalanobis distance and related statistical methods to DLS data.

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Stephen Dubin, Stanley Zietz, Karl L. Gabriel D.V.M., David Gabriel, Michael A. DellaVecchia M.D., and Rafat R. Ansari "Dynamic light scattering in veterinary medicine: refinement of diagnostic criteria", Proc. SPIE 4244, Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems XI, (21 May 2001); https://doi.org/10.1117/12.427768

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