Light-induced fluorescence for pulpal diagnosis

Arata Ebihara D.D.S.; Lih-Huei L. Liaw; Tatiana B. Krasieva; Petra B. B. Wilder-Smith

doi:10.1117/12.424495

27 April 2001 Light-induced fluorescence for pulpal diagnosis

Arata Ebihara D.D.S., Lih-Huei L. Liaw, Tatiana B. Krasieva, Petra B. B. Wilder-Smith

Proceedings Volume 4249, Lasers in Dentistry VII; (2001) https://doi.org/10.1117/12.424495
Event: BiOS 2001 The International Symposium on Biomedical Optics, 2001, San Jose, CA, United States

Abstract

A direct non-histological means of pulpal diagnosis remains elusive to clinical practice. Clinical vitality testing remains limited to electric, thermal criteria, or laser Doppler flowmetry. The goal of these investigations was to determine the feasibility of using light-induced fluorescence as a non-invasive modality for pulpal evaluation. Such a capability would, for example, permit expanded use of pulpotomy/pulpectomy techniques. Clinically healthy and diseased human extirpated pulpal tissues were used in this study. After excision, they were rapidly frozen and standard cryosections prepared. Measurement of tissue excitation/emission characteristics was performed using spectrographic analysis. A low-light level fluorescence microscopy system was then used to image autofluorescence localization and intensity at optimal excitation/detection parameters. Excitation/detection parameters used in this study included 405/605, 405/635, 405/670, 440/550, and 440/635. Autofluorescence intensities in healthy tissues were significantly stronger than those in diseased tissues at optimal parameters. It is postulated that autofluorescence characteristics are related to pathology- related structural changes in the pulp. This work provides the basis for further investigation into the relation between autofluorescence, histology and clinical symptoms.

Citation Download Citation

Arata Ebihara D.D.S., Lih-Huei L. Liaw, Tatiana B. Krasieva, and Petra B. B. Wilder-Smith "Light-induced fluorescence for pulpal diagnosis", Proc. SPIE 4249, Lasers in Dentistry VII, (27 April 2001); https://doi.org/10.1117/12.424495

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