Measurement of physiological parameters in tumors in vivo using MPLSM

Edward B. Brown III; Robert B. Campbell; Yoshikazu Tsuzuki; Dai Fukumura; Rakesh K. Jain

doi:10.1117/12.424547

24 April 2001 Measurement of physiological parameters in tumors in vivo using MPLSM

Edward B. Brown III, Robert B. Campbell, Yoshikazu Tsuzuki, Dai Fukumura, Rakesh K. Jain

Proceedings Volume 4262, Multiphoton Microscopy in the Biomedical Sciences; (2001) https://doi.org/10.1117/12.424547
Event: BiOS 2001 The International Symposium on Biomedical Optics, 2001, San Jose, CA, United States

Abstract

Conventional epifluorescence microscopy coupled with chronic animal window models has provided stunning insight into tumor pathophysiology, including gene expression, angiogenesis, interstitial transport, and drug delivery. However, the findings to date have been limited to the tumor surface (<150 microns). This is an important drawback because the internal architecture of tumors is known to be heterogeneous, with a collagenous tumor/host interface, highly vascularized outer regions, and poorly vascularized inner necrotic regions. Here we present the first application of the multiphoton laser-scanning microscope (MPLSM) to monitoring drug delivery in tumors, phenotypic tumor cell behavior, and tumor-induced gene promoter activity in vivo. Furthermore, we show that the MPLSM can be used in living tumors to quantify physiological parameters such as vascular density, blood flow velocity, leukocyte/endothelial interactions, and single vessel permeability. These measurements are performed with high three-dimensional resolution up to depths of several hundred microns, thus providing novel insights into the internal milieu of tumors. These findings will allow the development of drug therapeutic strategies that not only affect the tumor surface, but also internal regions.

Citation Download Citation

Edward B. Brown III, Robert B. Campbell, Yoshikazu Tsuzuki, Dai Fukumura, and Rakesh K. Jain "Measurement of physiological parameters in tumors in vivo using MPLSM", Proc. SPIE 4262, Multiphoton Microscopy in the Biomedical Sciences, (24 April 2001); https://doi.org/10.1117/12.424547

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available