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We have studied photodynamic action of He-Ne laser radiation on cultures of Staphylococcus (strain 209 P), Streptococcus anhaemolyticus, and total microflora of dental deposit been sensitized by methylene blue. The concentration of the dye was varied from 0.001% to 0.1%, radiation power density was 100 divided by 2300 mW/cm2. Irradiated strain was put into thermostat for 48 hours and the number of colonies was counted and analyzed.
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Ilya S. Ovchinnikov, Valery V. Tuchin, Krill I. Ivanov, Gennady M. Shoub, "Photodynamic action on some pathogenic microorganisms of oral cavity," Proc. SPIE 4422, Low-Level Laser Therapy, (26 April 2001); https://doi.org/10.1117/12.425520