In vivo and in vitro study of immersion clearing dynamics of the skin

Elina A. Genina; Alexey N. Bashkatov; Yurii P. Sinichkin; Nina A. Lakodina; Irina V. Korovina; Georgy V. Simonenko; Valery V. Tuchin

doi:10.1117/12.447123

31 October 2001 In vivo and in vitro study of immersion clearing dynamics of the skin

Elina A. Genina, Alexey N. Bashkatov, Yurii P. Sinichkin, Nina A. Lakodina, Irina V. Korovina, Georgy V. Simonenko, Valery V. Tuchin

Author Affiliations +

Proceedings Volume 4432, Diagnostic Optical Spectroscopy in Biomedicine; (2001) https://doi.org/10.1117/12.447123
Event: European Conferences on Biomedical Optics, 2001, Munich, Germany

Abstract

Experimental results on in vivo and in vitro immersion clearing dynamics of the skin under action of the osmotic agent are presented. The significant decrease of the human skin reflectance in vivo under action of glucose solution is demonstrated. In vitro experiments with dyed immersion agents have shown that the immersion mostly penetrates into skin tissue though the dermal layer. Diffusion of the immersion agent through the stratum corneum barrier was insignificant. It was shown the hypodermic injection of the immersion agent is an effective way for the agent administration. The time-dependent contrast of the blood perfused areas in the cleared skin was estimated. The diffusion coefficient of 40%-glucose solution in skin in vivo was estimated as D equals (2.56+/- 0.13)(DOT)10^-6cm²sec.

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Elina A. Genina, Alexey N. Bashkatov, Yurii P. Sinichkin, Nina A. Lakodina, Irina V. Korovina, Georgy V. Simonenko, and Valery V. Tuchin "In vivo and in vitro study of immersion clearing dynamics of the skin", Proc. SPIE 4432, Diagnostic Optical Spectroscopy in Biomedicine, (31 October 2001); https://doi.org/10.1117/12.447123

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