Analyzing LED-induced haemal fluorescent spectra on laboratory small albino rat

Shumei Gao; Xiaosen Luo; Xiufeng Lan; Fangxiang Jiao; Jian Lu; Xiaowu Ni; Jiaying Xu; Shiyue Lu; Jian Shen; Jiangang Liu

doi:10.1117/12.462534

12 April 2002 Analyzing LED-induced haemal fluorescent spectra on laboratory small albino rat

Shumei Gao, Xiaosen Luo, Xiufeng Lan, Fangxiang Jiao, Jian Lu, Xiaowu Ni, Jiaying Xu, Shiyue Lu, Jian Shen, Jiangang Liu

Author Affiliations +

Proceedings Volume 4536, International Workshop on Photonics and Imaging in Biology and Medicine; (2002) https://doi.org/10.1117/12.462534
Event: International Workshop on Photonics and Imaging in Biology and Medicine, 2001, Wuhan, China

Abstract

Native fluorescence spectral characteristics of red blood cells were studied in the visible region in this paper. Blood samples were collected from normal small albino rats. Native fluorescence spectra of the erythrocyte were induced using Light Emitting Diode (LED) at yellow wavelength about 570+/- 16 nm ((Delta) (lambda) _0.5approximately equals 32nm). As the rat's erythrocyte content of in physiological water is increasing, the fluorescent primary emission peak is red shifted from 588 nm to above 615 nm. Furthermore, the fluorescence intensity at about 600 nm was found to be maximal while the rat's erythrocyte consistence is 1%. Moreover, it is shown in large numbers of experiments that LED-induced fluorescence spectra of the erythrocyte are similar with the whole blood. It may make sense for low- intensity light therapy.

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Shumei Gao, Xiaosen Luo, Xiufeng Lan, Fangxiang Jiao, Jian Lu, Xiaowu Ni, Jiaying Xu, Shiyue Lu, Jian Shen, and Jiangang Liu "Analyzing LED-induced haemal fluorescent spectra on laboratory small albino rat", Proc. SPIE 4536, International Workshop on Photonics and Imaging in Biology and Medicine, (12 April 2002); https://doi.org/10.1117/12.462534

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KEYWORDS

Luminescence

Blood

Light emitting diodes

Laser induced fluorescence

Spectroscopy

In vivo imaging

Tissues

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