High-precision measurements in an optical tweezers for studying single-biomolecule motion

H. T. Chen; Y. M. Li; L. R. Lou; H. W. Wang

doi:10.1117/12.462528

12 April 2002 High-precision measurements in an optical tweezers for studying single-biomolecule motion

H. T. Chen, Y. M. Li, L. R. Lou, H. W. Wang

Proceedings Volume 4536, International Workshop on Photonics and Imaging in Biology and Medicine; (2002) https://doi.org/10.1117/12.462528
Event: International Workshop on Photonics and Imaging in Biology and Medicine, 2001, Wuhan, China

Abstract

Based on the micron precision manipulation and measurement in the optical tweezers, we built an optical-trap system that can make quantitative measurement of displacement with nanometer resolution in millisecond time scale and then we can measure the piconewton force involved in the process. It is an eminent tool for manipulating biological specimens at the macromolecular level. In this paper we describe its technical properties, design, and make some discussion.

Citation Download Citation

H. T. Chen, Y. M. Li, L. R. Lou, and H. W. Wang "High-precision measurements in an optical tweezers for studying single-biomolecule motion", Proc. SPIE 4536, International Workshop on Photonics and Imaging in Biology and Medicine, (12 April 2002); https://doi.org/10.1117/12.462528

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