Imaging of green fluorescent protein in live plant by scanning near-field optical microscopy

Jianhua Xu; Tao Chen; Jialin Sun; Jihua Guo; Jun Zhao

doi:10.1117/12.462571

12 April 2002 Imaging of green fluorescent protein in live plant by scanning near-field optical microscopy

Jianhua Xu, Tao Chen, Jialin Sun, Jihua Guo, Jun Zhao

Proceedings Volume 4536, International Workshop on Photonics and Imaging in Biology and Medicine; (2002) https://doi.org/10.1117/12.462571
Event: International Workshop on Photonics and Imaging in Biology and Medicine, 2001, Wuhan, China

Abstract

An auxin/IAA induced in vivo green fluorescent protein (GFP) in a living plant Arabidopsis root has been studied by a scanning near-field microscope in transmission mode. The promising near-field images of the inducible GFPs at sub- surface of a plant cell suggest that they may locate proximity to the cell wall, i.e. both sides of and in the cytoplasm membrane. The clear and faint fluorescent spots with 1-3 micrometers showed that the proteins localized nearer and farther to the cell wall, respectively. All GFP molecules gathered together in a cell, and no individual GFP was observed in the experiment.

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Jianhua Xu, Tao Chen, Jialin Sun, Jihua Guo, and Jun Zhao "Imaging of green fluorescent protein in live plant by scanning near-field optical microscopy", Proc. SPIE 4536, International Workshop on Photonics and Imaging in Biology and Medicine, (12 April 2002); https://doi.org/10.1117/12.462571

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KEYWORDS

Green fluorescent protein

Near field scanning optical microscopy

Proteins

In vivo imaging

Microscopes

Near field optics

Optical microscopy

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