Further study of trichosanthin's effect on mouse embryos with confocal laser scanning microscopy

Hui Xu; Chunyang Zhang; Hui Ma; Die Yan Chen

doi:10.1117/12.441372

25 September 2001 Further study of trichosanthin's effect on mouse embryos with confocal laser scanning microscopy

Hui Xu, Chunyang Zhang, Hui Ma, Die Yan Chen

Proceedings Volume 4548, Multispectral and Hyperspectral Image Acquisition and Processing; (2001) https://doi.org/10.1117/12.441372
Event: Multispectral Image Processing and Pattern Recognition, 2001, Wuhan, China

Abstract

Trichosanthin(TCS), a ribosome inactivating protein extracted from the root tuber of a traditional Chinese medicine herb Tian Huo Fen(THF), possessed abortifacient, anti-tumor and anti-human immunodeficiency virus(HIV) activities. For centuries in China, THF has been used as an effective folk medicine to terminate early and midtrimester pregnancies and to treat ectopic pregnancies, hydatidiform moles and trophoblastic tumor. We observed the changes in reactive oxygen species and intracellular calcium in mouse embryos induced by TCS with confocal laser scanning microscopy in combination with the fluorescene diacetate (DCFHDA) and Fluo-3-AM. The results indicated that TCS induced increase in intracellular calcium and production of reactive oxygen species in mouse embryos , and TCS inhibited the development of mouse embryos effectively. Mouse embryos of different developmental stages before implantation are used in the experiments. This provides new insight into mechanism for abortifacient activity of TCS.

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Hui Xu, Chunyang Zhang, Hui Ma, and Die Yan Chen "Further study of trichosanthin's effect on mouse embryos with confocal laser scanning microscopy", Proc. SPIE 4548, Multispectral and Hyperspectral Image Acquisition and Processing, (25 September 2001); https://doi.org/10.1117/12.441372

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KEYWORDS

Calcium

Confocal laser scanning microscopy

Oxygen

Luminescence

Confocal microscopy

Medicine

Molecular lasers

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