Characterization of biofilm components using a fiber optic spectrometer

Markus Leitz; R. Sekar; T. Griebe; S. Krautwald; Hilmar Franke

doi:10.1117/12.463820

26 March 2002 Characterization of biofilm components using a fiber optic spectrometer

Markus Leitz, R. Sekar, T. Griebe, S. Krautwald, Hilmar Franke

Proceedings Volume 4616, Optical Fibers and Sensors for Medical Applications II; (2002) https://doi.org/10.1117/12.463820
Event: International Symposium on Biomedical Optics, 2002, San Jose, CA, United States

Abstract

A simple technique for the optical characterization and detection of early stages of biofilm is presented. A bacterial biofilm was developed in a microcell, consisting of of two glass slides and the biofilm between the slides. This technique allowed the measurement of biofilm thickness and refractive index of the medium as a function of position and time. Using a sensititive broad bandgap foil as surface, the early stage of biofilm formation was investigated in a simple reflectivity measurement using a fiber optic spectrometer. The optical results were compared with the measurements of confocal laser scanning microscope (CLSM). The fiber optic spectrometer technique was suitable for the detection of low bacterial densities (10⁴ cm^-2).

Citation Download Citation

Markus Leitz, R. Sekar, T. Griebe, S. Krautwald, and Hilmar Franke "Characterization of biofilm components using a fiber optic spectrometer", Proc. SPIE 4616, Optical Fibers and Sensors for Medical Applications II, (26 March 2002); https://doi.org/10.1117/12.463820

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