Programmable beam-splitter for confocal laser scanning microscopy

Holger Birk; Johann Engelhardt; Rafael Storz; Nicole Hartmann; Joachim Bradl; Heinrich Ulrich

doi:10.1117/12.467841

15 May 2002 Programmable beam-splitter for confocal laser scanning microscopy

Holger Birk, Johann Engelhardt, Rafael Storz, Nicole Hartmann, Joachim Bradl, Heinrich Ulrich

Proceedings Volume 4621, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing IX; (2002) https://doi.org/10.1117/12.467841
Event: International Symposium on Biomedical Optics, 2002, San Jose, CA, United States

Abstract

In confocal microscopy manifold combinations of dyes are used. The entire wavelength range from near-ultraviolet to near-infrared is used to excite these dyes. Their emission is then detected primarily in the visible range. In addition to the number of dyes, the number of possible excitation laser lines is already large and constantly increasing. Due to the almost unlimited number of possible combinations, programmable devices are required. This is true for excitation-modules, beam-splitters and detection modules. Systems using filters with fixed spectral properties can practically fulfill the requirements for only a very small subset of applications. Programmable devices have been realized for excitation using Acousto Optical Tunable Filters (AOTF's). Freely definable spectral detectors are available for a perfect adaptation to the desired emission bands. The missing link for a completely filter-free design is now introduced using an Acousto Optical Beam Splitter (AOBS).

Citation Download Citation

Holger Birk, Johann Engelhardt, Rafael Storz, Nicole Hartmann, Joachim Bradl, and Heinrich Ulrich "Programmable beam-splitter for confocal laser scanning microscopy", Proc. SPIE 4621, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing IX, (15 May 2002); https://doi.org/10.1117/12.467841

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