Fiber-optic-based optical trapping and detection for lab-on-a-chip (LOC) applications

Cynthia Jensen McMullin; Amy A. Au; Jerome Quinsaat; Edward R. Lyons; Henry P. Lee

doi:10.1117/12.468343

28 May 2002 Fiber-optic-based optical trapping and detection for lab-on-a-chip (LOC) applications

Cynthia Jensen McMullin, Amy A. Au, Jerome Quinsaat, Edward R. Lyons, Henry P. Lee

Proceedings Volume 4622, Optical Diagnostics of Living Cells V; (2002) https://doi.org/10.1117/12.468343
Event: International Symposium on Biomedical Optics, 2002, San Jose, CA, United States

Abstract

A three-fiber optical trapping/detection system has been molded in poly(dimethyl siloxane) (PDMS) using anisotropically etched Si V-grooves as the primary or master mold. The process of reverse molding in PDMS maintains the benefits of fiber optic self-alignment previously used in Si V-grooves. Two, pigtailed laser diodes emitting at 830 nm and 980 nm are connected to cleaved, single-mode (SM), counter-propagating fibers, used for trapping polystyrene beads. Orthogonal to the trapping fibers is a multi mode detection fiber coupled to a spectrometer. Chemically treated beads trapped by the 830 and 980 nm diode lasers were excited using a 660 nm diode laser. By utilizing the optical clarity of PDMS, the fourth excitation source fiber is mounted below the PDMS trap and used to excite the trapped beads. Changes in the relative intensity of the trapping light are used to indicate the capture and position of a bead in the trap. Additionally, detection of the excitation source and bead fluorescence is monitored.

Citation Download Citation

Cynthia Jensen McMullin, Amy A. Au, Jerome Quinsaat, Edward R. Lyons, and Henry P. Lee "Fiber-optic-based optical trapping and detection for lab-on-a-chip (LOC) applications", Proc. SPIE 4622, Optical Diagnostics of Living Cells V, (28 May 2002); https://doi.org/10.1117/12.468343

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