Feasibility of an online fluorescence-based optical sensor for oxygen monitoring in cell-culture media

D. Patrick O'Neal; Michael Adam Meledeo; Michael V. Pishko; Gerard L. Cote

doi:10.1117/12.468311

23 May 2002 Feasibility of an online fluorescence-based optical sensor for oxygen monitoring in cell-culture media

D. Patrick O'Neal, Michael Adam Meledeo, Michael V. Pishko, Gerard L. Cote

Proceedings Volume 4624, Optical Diagnostics and Sensing of Biological Fluids and Glucose and Cholesterol Monitoring II; (2002) https://doi.org/10.1117/12.468311
Event: International Symposium on Biomedical Optics, 2002, San Jose, CA, United States

Abstract

Low molecular weight molecules are typically very difficult to detect directly in solution using commercially available SPR (surface plasmon resonance) instruments. This is because the mass change on binding is not sufficient to cause a detectable change in refractive index on binding to surface- bound receptors (e.g., antibodies). Some receptors, however, undergo extensive changes in tertiary structure upon binding ligands. Here we present data suggesting conformational changes in surface-bound receptors such as periplasmic binding proteins and calcium-binding proteins can be detected by SPR. This SPR response can be used to monitor specific binding of carbohydrates and calcium even though the molecular weight of these analytes would be difficult to detect using traditional SPR methods. Therefore this approach has potential applications for developing optical biosensors for such small molecules.

Citation Download Citation

D. Patrick O'Neal, Michael Adam Meledeo, Michael V. Pishko, and Gerard L. Cote "Feasibility of an online fluorescence-based optical sensor for oxygen monitoring in cell-culture media", Proc. SPIE 4624, Optical Diagnostics and Sensing of Biological Fluids and Glucose and Cholesterol Monitoring II, (23 May 2002); https://doi.org/10.1117/12.468311

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