Digital holography applied to microscopy

Christian D. Depeursinge; Etienne Cuche; Pierre Marquet; Tristan Colomb; Pia Dahlgren; Anca A. Marian; Frederic Montfort; Pierre J. Magistretti

doi:10.1117/12.469267

3 June 2002 Digital holography applied to microscopy

Christian D. Depeursinge, Etienne Cuche, Pierre Marquet, Tristan Colomb, Pia Dahlgren, Anca A. Marian, Frederic Montfort, Pierre J. Magistretti

Author Affiliations +

Proceedings Volume 4659, Practical Holography XVI and Holographic Materials VIII; (2002) https://doi.org/10.1117/12.469267
Event: Electronic Imaging, 2002, San Jose, California, United States

Abstract

We report on new developments of a technique called Digital Holographic Microscopy (DHM), for the numerical reconstruction of digital holograms taken in microscopy, which allows simultaneous amplitude and quantitative phase contrast imaging. The reconstruction method computes the propagation of the complex optical wavefront diffracted by the object and is used to determine the refractive index and/or shape of the object with accuracies in the nanometer range along the optical axis.. The method requires the acquisition of a single hologram. The technique comprises the recording of a digital hologram of the specimen by means of a standard CCD camera at the exit of a Mach-Zehnder or Michelson type interferometer. The quantitative nature of the reconstructed phase distribution has been demonstrated by an application to surface profilometry where step height differences of a few nanometers have been measured. Another application takes place in biology for transmission phase- contrast imaging of living cells in culture. The resolution for thickness measurements depends on the refractive index of the specimen and a resolution of approximately 30 nanometers in height, and about half of a micro in width, has been achieved for living neural cells in cultures by using a high numerical aperture.

Citation Download Citation

Christian D. Depeursinge, Etienne Cuche, Pierre Marquet, Tristan Colomb, Pia Dahlgren, Anca A. Marian, Frederic Montfort, and Pierre J. Magistretti "Digital holography applied to microscopy", Proc. SPIE 4659, Practical Holography XVI and Holographic Materials VIII, (3 June 2002); https://doi.org/10.1117/12.469267

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available