Whole-body fluorescent imaging of tumor cells transfected with EGFP

Ying Jin; Da Xing

doi:10.1117/12.482954

12 September 2002 Whole-body fluorescent imaging of tumor cells transfected with EGFP

Ying Jin, Da Xing

Proceedings Volume 4916, Optics in Health Care and Biomedical Optics: Diagnostics and Treatment; (2002) https://doi.org/10.1117/12.482954
Event: Photonics Asia, 2002, Shanghai, China

Abstract

The green fluorescent protein (GFP), from the bioluminescent jellyfish Aequorea victoria, yields a bright green fluorescence when expressed in either eukaryotic or prokaryotic cells and illuminated by blue or UV light. The characteristic properties of GFP make this protein a good candidate for use as a molecular reporter to monitor pattems of protein localization, gene expression, and intracellular protein trafficking in living cells. In this study, human lung cancer cells (ASTC-a-l) were infected with EGFP plasmid vectors, and incubated in RPMI 1640 culture medium supplemented with 15% FCS and 800tg/ml G4l8. Five cell clones were observed strong fluorescence after several generations. The flow cytometry was used to determine the GFP expression stability, the results showed that there was significant difference between 2#(3#) and 4#(5#) (P<0.0l). The nude mouse, 6 weeks of age, was injected s.c. with a single dose of 3x 106 infected tumor cells. The GFP fluorescence was directly exited by 488nm argon ion laser and recorded by digital camera with 530nm long pass filter. This new metastatic model can play a critical role in the study of the mechanism in lung cancer and in screening of therapeutics that prevent or reverse this process. Key Words: GFP; Human lung cancer cells (ASTC-a-l); Flow cytometry; Nude mouse

Citation Download Citation

Ying Jin and Da Xing "Whole-body fluorescent imaging of tumor cells transfected with EGFP", Proc. SPIE 4916, Optics in Health Care and Biomedical Optics: Diagnostics and Treatment, (12 September 2002); https://doi.org/10.1117/12.482954

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