Two-photon fluorescence imaging of embryo with much less damage than confocal imaging

Bian Liu; Hui Xu; Lei Jin; Hui Ma; Die Yan Chen

doi:10.1117/12.465727

20 September 2002 Two-photon fluorescence imaging of embryo with much less damage than confocal imaging

Bian Liu, Hui Xu, Lei Jin, Hui Ma, Die Yan Chen

Proceedings Volume 4919, Advanced Materials and Devices for Sensing and Imaging; (2002) https://doi.org/10.1117/12.465727
Event: Photonics Asia, 2002, Shanghai, China

Abstract

Two-photon Laser Scanning Microscopy (TPLSM) is a novel technique based on the two-photon excitation of fluorophore. In this paper, TPLSM and traditional confocal microscopy are introduced. And the influence of femtosecond near-infrared (NIR) illumination on mouse embryos is investigated for the first time. The result shows that NIR laser has much less damage to embryos than blue laser and proves that TPLSM is superior to conventional confocal microscopy in keeping sample alive. TPLSM enables us to make a continuous observation for a longer time on embryogenesis.

Citation Download Citation

Bian Liu, Hui Xu, Lei Jin, Hui Ma, and Die Yan Chen "Two-photon fluorescence imaging of embryo with much less damage than confocal imaging", Proc. SPIE 4919, Advanced Materials and Devices for Sensing and Imaging, (20 September 2002); https://doi.org/10.1117/12.465727

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available