Amplification and modeling of bioaffinity detection with terahertz spectroscopy

Samuel P. Mickan; Abdellah Menikh; Jesper Munch; Derek Abbott; Xi-Cheng Zhang

doi:10.1117/12.488143

14 November 2002 Amplification and modelling of bioaffinity detection with terahertz spectroscopy

Samuel P. Mickan, Abdellah Menikh, Jesper Munch, Derek Abbott, Xi-Cheng Zhang

Proceedings Volume 4937, Biomedical Applications of Micro- and Nanoengineering; (2002) https://doi.org/10.1117/12.488143
Event: SPIE's International Symposium on Smart Materials, Nano-, and Micro- Smart Systems, 2002, Melbourne, Australia

Abstract

A new bioaffinity sensor based on pulsed terahertz (THz) spectroscopy is able to sensitively detect the presence of ultra-thin bound biomolecular layers. The protein avidin and lipid biotin are noted for their very high binding affinity, and the ease for which they can be attached to residues with importance in many biosensing applications. We demonstrate the sensitivity of the pulsed THz spectrometer to thin avidin layers and to avidin amplified with micron-sized agarose beads. The experimental results can be simply modelled by considering transmission of the THz radiation at the thin film interfaces. We detect less than 10.3 ng/cm² avidin, giving the THz system a detection capability of sub-thin solid films better than ellipsometry and reflectometry techniques.

Citation Download Citation

Samuel P. Mickan, Abdellah Menikh, Jesper Munch, Derek Abbott, and Xi-Cheng Zhang "Amplification and modelling of bioaffinity detection with terahertz spectroscopy", Proc. SPIE 4937, Biomedical Applications of Micro- and Nanoengineering, (14 November 2002); https://doi.org/10.1117/12.488143

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available