Estimation of optical fluorescent impulse response kernel in combined time and wavelength spaces

Olga V. Ivanova; Laura Marcu; Michael C. K. Khoo

doi:10.1117/12.478213

29 July 2003 Estimation of optical fluorescent impulse response kernel in combined time and wavelength spaces

Olga V. Ivanova, Laura Marcu, Michael C. K. Khoo

Proceedings Volume 4955, Optical Tomography and Spectroscopy of Tissue V; (2003) https://doi.org/10.1117/12.478213
Event: Biomedical Optics, 2003, San Jose, CA, United States

Abstract

Current state of the knowledge in the field of optical diagnostics indicates that application of both steady state and time-resolved fluorescence spectroscopy permits the discrimination among normal and pathological tissues. Most data analysis algorithms, however, separately address either the spectral profile of the fluorescence emission or the time domain features of fluorescence decay of tissue sample. We present a novel system identification technique that allows for intrinsic Fluorescent Impulse Response Kernel (FIRK) estimation in combined time and wavelength spaces. The fluorescence experiment is described as a discrete two-dimensional single input - single output system. Modeling of FIRK is based on the characteristics of spectro-temporal fluorescence waveforms. Taking advantage of the exponentially decaying temporal behavior of the fluorescent output, we use a linear combination of Laguerre functions to model the fluorescence response as a function of time. To address the large variability of spectral profiles from different biological samples, we use the discrete Fourier series expansion to model the variation of fluorescence intensity across wavelength. The validation of the proposed approach is performed on simulated fluorescence data and fluorescence data measured from fluorescence lifetime standards. The algorithm is applicable for an effective recovery of intrinsic FIRK from any excitation light probe spectro-temporal waveforms. The retrieved FIRK can be further used for modeling and/or classification of pathological and physiological conditions of tissues.

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Olga V. Ivanova, Laura Marcu, and Michael C. K. Khoo "Estimation of optical fluorescent impulse response kernel in combined time and wavelength spaces", Proc. SPIE 4955, Optical Tomography and Spectroscopy of Tissue V, (29 July 2003); https://doi.org/10.1117/12.478213

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KEYWORDS

Luminescence

Data modeling

Tissues

Bioalcohols

System identification

Signal processing

Statistical analysis

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