Molecular contrast in optical coherence tomography using a pump-probe technique

K. Divikara Rao; Michael A. Choma; Siavash Yazdanfar; Andrew M. Rollins; Joseph A. Izatt

doi:10.1117/12.479039

8 July 2003 Molecular contrast in optical coherence tomography using a pump-probe technique

K. Divikara Rao, Michael A. Choma, Siavash Yazdanfar, Andrew M. Rollins, Joseph A. Izatt

Proceedings Volume 4956, Coherence Domain Optical Methods and Optical Coherence Tomography in Biomedicine VII; (2003) https://doi.org/10.1117/12.479039
Event: Biomedical Optics, 2003, San Jose, CA, United States

Abstract

Optical coherence tomography (OCT) is an emerging tool for real time in-situ tissue imaging with micrometer-scale resolution. Real-time OCT systems have been integrated into clinical medical diagnostics, and functional extensions such as polarization-sensitive, Doppler, and spectroscopic OCT have recently been introduced. However, OCT imaging technology has not yet taken advantage of molecular-specific contrast mechanisms which have revolutionized other medical and biological imaging modalities such as MRI, PET, SPECT, and fluorescence microscopy. OCT is by definition insensitive to incoherent scattering processes such fluorescence or spontaneous Raman scattering. We have previously proposed the use of spectroscopic OCT for detection of light from coherent inelastic scattering processes such as stimulated emission, stimulate Raman scattering, and other four-wave mixing processes. In this report we present a new technique which we call pump probe OCT (PPOCT), which is a novel extension of OCT for enhancing the contrast of OCT images based on transient absorption in the sample induced by an external pump beam. We show preliminary data for PPOCT using methylene blue as a molecular contrast agent and show depth resolved PPOCT M-scans that localize the presence of dye in a well phantom.

Citation Download Citation

K. Divikara Rao, Michael A. Choma, Siavash Yazdanfar, Andrew M. Rollins, and Joseph A. Izatt "Molecular contrast in optical coherence tomography using a pump-probe technique", Proc. SPIE 4956, Coherence Domain Optical Methods and Optical Coherence Tomography in Biomedicine VII, (8 July 2003); https://doi.org/10.1117/12.479039

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