Optical tweezers for the measurement of binding forces: system description and application for the study of E. coli adhesion

Erik G. Fallman; Staffan Schedin; Magnus J. Andersson; Jana Jass; Ove Axner

doi:10.1117/12.504304

19 June 2003 Optical tweezers for the measurement of binding forces: system description and application for the study of E. coli adhesion

Erik G. Fallman, Staffan Schedin, Magnus J. Andersson, Jana Jass, Ove Axner

Author Affiliations +

Proceedings Volume 4962, Manipulation and Analysis of Biomolecules, Cells, and Tissues; (2003) https://doi.org/10.1117/12.504304
Event: Biomedical Optics, 2003, San Jose, CA, United States

Abstract

Optical tweezers together with a position sensitive detection system allows measurements of forces in the pN range between micro-sized biological objects. A prototype force measurement system has been constructed around in inverted microscope with an argon-ion pumped Ti:sapphire laser as light source for optical trapping. A trapped particle in the focus of the high numerical aperture microscope-objective behaves like an omni-directional mechanical spring if an external force displaces it. The displacement from the equilibrium position is a measure of the exerted force. For position detection of the trapped particle (polystyrene beads), a He-Ne laser beam is focused a small distance below the trapping focus. An image of the bead appears as a distinct spot in the far field, monitored by a photosensitive detector. The position data is converted to a force measurement by a calibration procedure. The system has been used for measuring the binding forces between E-coli bacterial adhesin and their receptor sugars.

Citation Download Citation

Erik G. Fallman, Staffan Schedin, Magnus J. Andersson, Jana Jass, and Ove Axner "Optical tweezers for the measurement of binding forces: system description and application for the study of E. coli adhesion", Proc. SPIE 4962, Manipulation and Analysis of Biomolecules, Cells, and Tissues, (19 June 2003); https://doi.org/10.1117/12.504304

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