Mean cell size and collagen orientation from 2D Fourier analysis of confocal laser scanning microscopy and two-photon fluorescence microscopy on human skin in vivo

Gerald W. Lucassen; Bernard L.G. Bakker; Sieglinde Neerken; Rob F. M. Hendriks

doi:10.1117/12.478335

9 July 2003 Mean cell size and collagen orientation from 2D Fourier analysis on confocal laser scanning microscopy and two-photon fluorescence microscopy on human skin in vivo

Gerald W. Lucassen, Bernard L.G. Bakker, Sieglinde Neerken, Rob F. M. Hendriks

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Proceedings Volume 4964, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing X; (2003) https://doi.org/10.1117/12.478335
Event: Biomedical Optics, 2003, San Jose, CA, United States

Abstract

We present results from 2D Fourier analysis on 3D stacks of images obtained by confocal laser scanning reflectance microscopy (CLSM) and two-photon fluorescence microscopy (2PM) on human skin in vivo. CLSM images were obtained with a modified commercial system (Vivascope1000, Lucid Inc, excitation wavelength 830 nm) equipped with a piezo-focusing element (350 μm range) for depth positioning of the objective lens. 2PM was performed with a specially designed set-up with excitation wavelength 730 nm. Mean cell size in the epidermal layer and structural orientation in the dermal layer have been determined as a function of depth by 2D Fourier analysis. Fourier analysis on microscopic images enables automatic non-invasive quantitative structural analysis (mean cell size and orientation) of living human skin.

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Gerald W. Lucassen, Bernard L.G. Bakker, Sieglinde Neerken, and Rob F. M. Hendriks "Mean cell size and collagen orientation from 2D Fourier analysis on confocal laser scanning microscopy and two-photon fluorescence microscopy on human skin in vivo", Proc. SPIE 4964, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing X, (9 July 2003); https://doi.org/10.1117/12.478335

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KEYWORDS

Skin

In vivo imaging

Two photon excitation microscopy

Collagen

Confocal laser scanning microscopy

3D image processing

Confocal microscopy

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