Use of molecular beacons for the detection of bacteria in microfluidic devices

Chuanwu Xi; Stephen A. Boppart; Lutgarde Raskin

doi:10.1117/12.478143

17 January 2003 Use of molecular beacons for the detection of bacteria in microfluidic devices

Chuanwu Xi, Stephen A. Boppart, Lutgarde Raskin

Proceedings Volume 4982, Microfluidics, BioMEMS, and Medical Microsystems; (2003) https://doi.org/10.1117/12.478143
Event: Micromachining and Microfabrication, 2003, San Jose, CA, United States

Abstract

We present the use of DNA and peptide nucleic acid (PNA) molecular beacons (MBs) as sensitive indicators in microfluidic bioMEM devices. DNA and PNA MBs can be used to quantitatively study hybridization kinetics in real time in a polydimethyl siloxane (PDMS) microfluidic device. PNA MBs perform better than DNA MBs for the study of hybridization kinetics of rRNA targets in real time in microfluidic channels. We also demonstrate the use of PNA MBs for fast detection of bacterial cells in microfluidic channels. Using PNA MBs as detection probes will enable us to develop an integrated biosensor for the rapid and on-site detection and quantification of microbial pathogens in environmental and clinical samples.

Citation Download Citation

Chuanwu Xi, Stephen A. Boppart, and Lutgarde Raskin "Use of molecular beacons for the detection of bacteria in microfluidic devices", Proc. SPIE 4982, Microfluidics, BioMEMS, and Medical Microsystems, (17 January 2003); https://doi.org/10.1117/12.478143

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