Computer-aided photometric analysis of dynamic digital bioluminescent images

Zbigniew Gorski; T. Bembnista; J. Floryszak-Wieczorek; Marek Domanski; Janusz Slawinski

doi:10.1117/12.501362

1 April 2003 Computer-aided photometric analysis of dynamic digital bioluminescent images

Zbigniew Gorski, T. Bembnista, J. Floryszak-Wieczorek, Marek Domanski, Janusz Slawinski

Proceedings Volume 5064, Lightmetry 2002: Metrology and Testing Techniques Using Light; (2003) https://doi.org/10.1117/12.501362
Event: Lightmetry 2002, 2002, Warsaw, Poland

Abstract

The paper deals with photometric and morphologic analysis of bioluminescent images obtained by registration of light radiated directly from some plant objects. Registration of images obtained from ultra-weak light sources by the single photon counting (SPC) technique is the subject of this work. The radiation is registered by use of a 16-bit charge coupled device (CCD) camera "Night Owl" together with WinLight EG&G Berthold software. Additional application-specific software has been developed in order to deal with objects that are changing during the exposition time. Advantages of the elaborated set of easy configurable tools named FCT for a computer-aided photometric and morphologic analysis of numerous series of quantitatively imperfect chemiluminescent images are described. Instructions are given how to use these tools and exemplified with several algorithms for the transformation of images library. Using the proposed FCT set, automatic photometric and morphologic analysis of the information hidden within series of chemiluminescent images reflecting defensive processes in poinsettia (Euphorbia pulcherrima Willd) leaves affected by a pathogenic fungus Botrytis cinerea is revealed.

Citation Download Citation

Zbigniew Gorski, T. Bembnista, J. Floryszak-Wieczorek, Marek Domanski, and Janusz Slawinski "Computer-aided photometric analysis of dynamic digital bioluminescent images", Proc. SPIE 5064, Lightmetry 2002: Metrology and Testing Techniques Using Light, (1 April 2003); https://doi.org/10.1117/12.501362

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