Optical coherence tomography for visualization of plant tissues

Veronika V. Sapozhnikova; Vladislav A. Kamensky; Roman V. Kuranov

doi:10.1117/12.519766

22 September 2003 Optical coherence tomography for visualization of plant tissues

Veronika V. Sapozhnikova, Vladislav A. Kamensky, Roman V. Kuranov

Proceedings Volume 5149, Laser Applications in Medicine, Biology, and Environmental Science; (2003) https://doi.org/10.1117/12.519766
Event: International Conference on Lasers, Applications, and Technologies 2002, 2002, Moscow, Russian Federation

Abstract

First experimental results on optical coherence tomography (OCT) imaging of internal structure of plant tissues and in situ OCT monitoring of plant tissue regeneration at different water supply are reported. The OCT images are a map of backscattering coefficient 200 x 200 pixels, 1.5 mm along plant surface and up to 2 mm in depth (by analogy with ultrasound B-scan). Experiments for evaluating OCT capabilities were performed on Tradescantia blossfeldiana Mild. Using OCT device, in vivo images of plant tissues were acquired with a spatial resolution of 15 μm. These OCT images correlate with standard microscopy data from the same tissue regions. Since incident light power at a wavelength of 1.3 μm is as low as 0.5 mW, two-dimensional OCT images can be obtained directly on an intact plant not damaging it. Acquisition time for a 2D image is 1-3 s. OCT enables effective monitoring of fast reactions in plants at different water supply.

Citation Download Citation

Veronika V. Sapozhnikova, Vladislav A. Kamensky, and Roman V. Kuranov "Optical coherence tomography for visualization of plant tissues", Proc. SPIE 5149, Laser Applications in Medicine, Biology, and Environmental Science, (22 September 2003); https://doi.org/10.1117/12.519766

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available