Validation of a time-resolved fluorescence spectroscopy apparatus in a rabbit atherosclerosis model

Qiyin Fang; Javier A. Jo; Thanassis Papaioannou; Amir Dorafshar; Todd Reil; Jian-Hua Qiao; Michael C. Fishbein; Julie A. Freischlag; Laura Marcu

doi:10.1117/12.529677

13 July 2004 Validation of a time-resolved fluorescence spectroscopy apparatus in a rabbit atherosclerosis model

Qiyin Fang, Javier A. Jo, Thanassis Papaioannou, Amir Dorafshar, Todd Reil, Jian-Hua Qiao, Michael C. Fishbein, Julie A. Freischlag, Laura Marcu

Author Affiliations +

Proceedings Volume 5312, Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems XIV; (2004) https://doi.org/10.1117/12.529677
Event: Biomedical Optics 2004, 2004, San Jose, CA, United States

Abstract

Time-resolved laser-induced fluorescence spectroscopy (tr-LIFS) has been studied as a potential tool for in vivo diagnosis of atherosclerotic lesions. This study is to evaluate the potential of a compact fiber-optics based tr-LIFS instrument developed in our laboratory for in vivo analysis of atherosclerotic plaque composition. Time-resolved fluorescence spectroscopy studies were performed in vivo on fifteen New Zealand White rabbits (atherosclerotic: N=8, control: N=7). Time-resolved fluorescence spectra were acquired (range: 360-600 nm, increment: 5 nm, total acquisition time: 65 s) from normal aorta wall and lesions in the abdominal aorta. Data were analyzed in terms of fluorescence emission spectra and wavelength specific lifetimes. Following trichrome staining, tissue specimens were analyzed histopathologically in terms of intima/media thickness and biochemical composition (collagen, elastin, foam cells, and etc). Based on intimal thickness, the lesions were divided into thin and thick lesions. Each group was further separated into two categories: collagen rich lesions and foam cell rich lesions based on their biochemical composition. The obtained spectral and time domain fluorescence signatures were subsequently correlated to the histopathological findings. The results have shown that time-domain fluorescence spectral features can be used in vivo to separate atherosclerotic lesions from normal aorta wall as well discrimination within certain types of lesions.

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Qiyin Fang, Javier A. Jo, Thanassis Papaioannou, Amir Dorafshar, Todd Reil, Jian-Hua Qiao, Michael C. Fishbein, Julie A. Freischlag, and Laura Marcu "Validation of a time-resolved fluorescence spectroscopy apparatus in a rabbit atherosclerosis model", Proc. SPIE 5312, Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems XIV, (13 July 2004); https://doi.org/10.1117/12.529677

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KEYWORDS

Luminescence

Time resolved spectroscopy

In vivo imaging

Fiber optics

Foam

Data acquisition

Fluorescence spectroscopy

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