Active particle manipulation with four laser beams

Jan Huisken; Jim Swoger; Ernst H. K. Stelzer

doi:10.1117/12.529171

1 July 2004 Active particle manipulation with four laser beams

Jan Huisken, Jim Swoger, Ernst H. K. Stelzer

Proceedings Volume 5322, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues II; (2004) https://doi.org/10.1117/12.529171
Event: Biomedical Optics 2004, 2004, San Jose, CA, United States

Abstract

Trapping and active movement of the sample in a microscope is gently performed by applying optical forces. Optical tweezers, including the recently developed dynamic multiple-beam optical tweezers, have been shown to be indispensable tools in several fields. We demonstrate an optical trap that is formed by four laser beams. In our Differential Active Optical Manipulator (DAOM) the beams are arranged along the axes of a tetrahedron and are approximately collimated within the vicinity of their point of intersection. The DAOM makes use of a distinctive advantage of collimated light: in contrast to optical tweezers, this instrument allows movement of the confined particle over long distances without mechanical scanning. The beams' scattering forces superimpose in their common volume, and the total force (magnitude and direction) on the particle can be set by adjusting the beams' intensities. This can be done quickly and independently for each beam, allowing us to move a particle along arbitrary paths within a volume of about 1nℓ. Active feedback ensures that the sample can be held in place or transported to a specified position as desired. This allows focusing and lateral translation of the microscopic sample, which are common tasks in microscopy. During manipulation the particle is simultaneously observed from four sides. This opens new perspectives for 3D microscopy combined with contactless manipulation of the sample under observation.

Citation Download Citation

Jan Huisken, Jim Swoger, and Ernst H. K. Stelzer "Active particle manipulation with four laser beams", Proc. SPIE 5322, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues II, (1 July 2004); https://doi.org/10.1117/12.529171

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