In vitro gene transfer by the application of laser-induced stress wave: effect of cellular heating

Mitsuhiro Terakawa; Shunichi Sato; Makoto Ogura; Kuniaki Nakanishi; Yoshinori Masaki; Maki Uenoyama; Hitoshi Wakisaka; Hiroshi Ashida; Minoru Obara

doi:10.1117/12.530815

1 July 2004 In vitro gene transfer by the application of laser-induced stress wave: effect of cellular heating

Mitsuhiro Terakawa, Shunichi Sato, Makoto Ogura, Kuniaki Nakanishi, Yoshinori Masaki, Maki Uenoyama, Hitoshi Wakisaka, Hiroshi Ashida, Minoru Obara

Author Affiliations +

Proceedings Volume 5322, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues II; (2004) https://doi.org/10.1117/12.530815
Event: Biomedical Optics 2004, 2004, San Jose, CA, United States

Abstract

We previously demonstrated in vitro that the simultaneous application of cellular heating and a laser-induced stress wave (LISW) enhanced the uptake of porfimer sodium (Photofrin) by cells. In this study, we attempted to apply this technique to gene transfer to cultured cells. LISW and/or a transient mild (~43°C) heating been applied to deliver plasmid coding for green fluorescent protein (EGFP) to NIH-3T3 cells. It was found that simultaneous application of an LISW and the heating significantly increased the transfection efficiency by a factor of 2.5 when compared with that for the cells treated with an LISW alone.

Citation Download Citation

Mitsuhiro Terakawa, Shunichi Sato, Makoto Ogura, Kuniaki Nakanishi, Yoshinori Masaki, Maki Uenoyama, Hitoshi Wakisaka, Hiroshi Ashida, and Minoru Obara "In vitro gene transfer by the application of laser-induced stress wave: effect of cellular heating", Proc. SPIE 5322, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues II, (1 July 2004); https://doi.org/10.1117/12.530815

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