Automatic quantification of neurite outgrowth by means of image analysis

Gert Van de Wouwer; Rony Nuydens; Theo Meert; Barbara Weyn

doi:10.1117/12.525634

13 July 2004 Automatic quantification of neurite outgrowth by means of image analysis

Gert Van de Wouwer, Rony Nuydens, Theo Meert, Barbara Weyn

Proceedings Volume 5324, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XI; (2004) https://doi.org/10.1117/12.525634
Event: Biomedical Optics 2004, 2004, San Jose, CA, United States

Abstract

A system for quantification of neurite outgrowth in in-vitro experiments is described. The system is developed for routine use in a high-throughput setting and is therefore needs fast, cheap, and robust. It relies on automated digital microscopical imaging of microtiter plates. Image analysis is applied to extract features for characterisation of neurite outgrowth. The system is tested in a dose-response experiment on PC12 cells + Taxol. The performance of the system and its ability to measure changes on neuronal morphology is studied.

Citation Download Citation

Gert Van de Wouwer, Rony Nuydens, Theo Meert, and Barbara Weyn "Automatic quantification of neurite outgrowth by means of image analysis", Proc. SPIE 5324, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XI, (13 July 2004); https://doi.org/10.1117/12.525634

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