Development of a photoactivatable fluorescent protein from <i>Aequorea victoria</i> GFP

George H. Patterson; Jennifer Lippincott-Schwartz

doi:10.1117/12.532886

14 June 2004 Development of a photoactivatable fluorescent protein from Aequorea victoria GFP

George H. Patterson, Jennifer Lippincott-Schwartz

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Proceedings Volume 5329, Genetically Engineered and Optical Probes for Biomedical Applications II; (2004) https://doi.org/10.1117/12.532886
Event: Biomedical Optics 2004, 2004, San Jose, CA, United States

Abstract

Photoactivation, the rapid conversion of photoactivatable molecules to a fluorescent state by intense irradiation, can be used to mark and monitor selected molecules within cells¹. We report a photoactivatable variant of the Aequorea victoria green fluorescent protein (GFP) based on a mutation at position 203 that upon intense irradiation with 413 nm light exhibits a stable 60-100 fluorescence increase under 488 nm excitation. The photoactivated form of this mutant named photoactivatable GFP (PA-GFP), is stable under a number of conditions. PA-GFP can be used to analyze protein dynamics in living cells, offering enormous potential for addressing outstanding questions in protein trafficking and turnover, organelle dynamics, and cell lineage patterns.

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George H. Patterson and Jennifer Lippincott-Schwartz "Development of a photoactivatable fluorescent protein from Aequorea victoria GFP", Proc. SPIE 5329, Genetically Engineered and Optical Probes for Biomedical Applications II, (14 June 2004); https://doi.org/10.1117/12.532886

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