Optical tweezers study of viscoelastic properties in the outer hair cell plasma membrane

David R. Murdock; Sergey A. Ermilov; Feng Qian; William E. Brownell; Bahman Anvari

doi:10.1117/12.529597

1 June 2004 Optical tweezers study of viscoelastic properties in the outer hair cell plasma membrane

David R. Murdock, Sergey A. Ermilov, Feng Qian, William E. Brownell, Bahman Anvari

Proceedings Volume 5331, Nanobiophotonics and Biomedical Applications; (2004) https://doi.org/10.1117/12.529597
Event: Biomedical Optics 2004, 2004, San Jose, CA, United States

Abstract

An optical tweezers system was used to study the mechanical characteristics of the outer hair cell (OHC) lateral wall by forming plasma membrane tethers. A 2^nd order generalized Kelvin model was applied to describe the viscoelastic behavior of OHC membrane tethers. The measured parameters included equilibrium tethering force, (F_eq), force relaxation times (τ), stiffness values (κ), and coefficients of friction (μ). An analysis of force relaxation in membrane tethers indicated that the force decay is a biphasic process containing both an elastic and a viscous phase. In general, we observed an overall negative trend in the measured parameters upon application of the cationic amphipath chlorpromazine (CPZ). CPZ was found to cause up to a 40 pN reduction in F_eq in OHCs. A statistically significant reduction in relaxation times and coefficients of friction was also observed, suggesting an increase in rate of force decay and a decrease in plasma membrane viscosity.

Citation Download Citation

David R. Murdock, Sergey A. Ermilov, Feng Qian, William E. Brownell, and Bahman Anvari "Optical tweezers study of viscoelastic properties in the outer hair cell plasma membrane", Proc. SPIE 5331, Nanobiophotonics and Biomedical Applications, (1 June 2004); https://doi.org/10.1117/12.529597

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