Osteoblast adhesion of breast cancer cells with scanned image microscopy

Chiaki Miyasaka; Bernhard R. Tittmann; Robyn R. Mercer; Andrea M. Mastro

doi:10.1117/12.540003

21 July 2004 Osteoblast adhesion of breast cancer cells with scanned image microscopy

Chiaki Miyasaka, Bernhard R. Tittmann, Robyn R. Mercer, Andrea M. Mastro

Proceedings Volume 5394, Health Monitoring and Smart Nondestructive Evaluation of Structural and Biological Systems III; (2004) https://doi.org/10.1117/12.540003
Event: NDE for Health Monitoring and Diagnostics, 2004, San Diego, CA, United States

Abstract

Breast cancer frequently metastasizes, or spreads, to the bone. Upon colonizing bone tissue, the cancer cells stimulate osteoclasts (cells that break bone down), resulting in large lesions in the bone. It seems that breast cancer cells also affect osteoblasts (cells that build new bone), in addition to osteoclasts. To test this hypothesis, conditioned medium was collected from a bone-metastatic breast cancer cell line, MDA-MB-231, and then cultured with an immature osteoblast cell line, MC3T3-E1. To characterize cell adhesion, MC3T3-E1 osteoblasts were examined with a mechanical scanning acoustic reflection microscope (SAM). The cells were cultured with or without MDA-MB-231 conditioned medium for 2 days, and then assayed microscopically. The SAM indicated that MC3T3-E1 osteoblasts were firmly attached to their plastic substrate. However, MC3T3-E1 cells cultured with MDA-MB-231 conditioned medium displayed both an abnormal shape and poor adhesion at the substrate interface. These data taken together suggest that MDA-MB-231 breast cancer cells alter MC3T3-E1 adhesion. Using optical microscopic techniques, we were not able to observe these differences until the cells were quite confluent after 7 days of culture. However, using the SAM, we were able to detect these changes within 2 days of culture with MDA-MB-231 conditioned medium.

Citation Download Citation

Chiaki Miyasaka, Bernhard R. Tittmann, Robyn R. Mercer, and Andrea M. Mastro "Osteoblast adhesion of breast cancer cells with scanned image microscopy", Proc. SPIE 5394, Health Monitoring and Smart Nondestructive Evaluation of Structural and Biological Systems III, (21 July 2004); https://doi.org/10.1117/12.540003

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