Protein aggregation processes in solutions containing heavy metal and chelate ions: studying by laser light scattering and polarized fluorescence methods

Galina P. Petrova; Yurii M. Petrusevich; Natalie V. Sokol; Dmitrii I. Ten

doi:10.1117/12.571379

29 July 2004 Protein aggregation processes in solutions containing heavy metal and chelate ions: studying by laser light scattering and polarized fluorescence methods

Galina P. Petrova, Yurii M. Petrusevich, Natalie V. Sokol, Dmitrii I. Ten

Author Affiliations +

Proceedings Volume 5486, ALT'03 International Conference on Advanced Laser Technologies: Biomedical Optics; (2004) https://doi.org/10.1117/12.571379
Event: ALT'03 International Conference on Advanced Laser Technologies: Biomedical Optics, 2003, Silsoe, United Kingdom

Abstract

The formation of macromolecular protein clusters in the presence of some heavy ions was observed by the Rayleigh light scattering and polarized fluorescence methods. Luminescence spectra of europium chelate and BAS-europium chelate water solutions were obtained at different pH and concentrations of components. The rotational correlation time of particles in the solutions containing heavy ions increase significantly in comparsion with rotational correlation time of protein macromolecule. This result can be explained by macromolecule clusters formation.

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Galina P. Petrova, Yurii M. Petrusevich, Natalie V. Sokol, and Dmitrii I. Ten "Protein aggregation processes in solutions containing heavy metal and chelate ions: studying by laser light scattering and polarized fluorescence methods", Proc. SPIE 5486, ALT'03 International Conference on Advanced Laser Technologies: Biomedical Optics, (29 July 2004); https://doi.org/10.1117/12.571379

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