A novel in vitro and in situ immunoassay biosensor based on fiber optic Fabry-Perot interferometry

Chun-Jun Lin; Yuan-Tai Tseng; Shih-Chang Lin; Chung-Shi Yang; Fan-Gang Tseng

doi:10.1117/12.566616

9 June 2004 A novel in vitro and in situ immunoassay biosensor based on fiber optic Fabry-Perot interferometry

Chun-Jun Lin, Yuan-Tai Tseng, Shih-Chang Lin, Chung-Shi Yang, Fan-Gang Tseng

Proceedings Volume 5502, Second European Workshop on Optical Fibre Sensors; (2004) https://doi.org/10.1117/12.566616
Event: Second European Workshop on Optical Fibre Sensors, 2004, Santander, Spain

Abstract

This paper proposes a novel fiber optic immunoassay biosensor based on the Fabry-Perot (F-P) interferometry. The PDMS dip coating and self-assembly monolayers coating are employed to prepare the fiber probes in the formation of F-P cavity and modification of the tip surface respectively. As the fiber probe inserting into the target solution, the immunoreaction will be introduced on the fiber tip. The covalent binding of the Rabbit IgG and Anti Rabbit IgG-Cy3 molecules on the fiber tip will contribute to the variation of the refractive index of interface as well as the reflectance. The interference spectrum will be shifted due to the reflectance variation. The time response is also implemented by taking the in-situ measurement of spectrum valley shift and the signal will reach the steady state within 15 minutes. Finally, the sensitivity of this immunoassay biosensor has also been demonstrated that the lowest detectable concentration of the target sample Anti Rabbit IgG-Cy3 is 10^-12 g/ml, which is lower than the detection limit of ELISA of 5*10^-11 g/ml. This fiber optic immunoassay biosensor will be applied in the brain resarch for in-vivo and in-situ monitoring the biochemical reactions of the neurocytes.

Citation Download Citation

Chun-Jun Lin, Yuan-Tai Tseng, Shih-Chang Lin, Chung-Shi Yang, and Fan-Gang Tseng "A novel in vitro and in situ immunoassay biosensor based on fiber optic Fabry-Perot interferometry", Proc. SPIE 5502, Second European Workshop on Optical Fibre Sensors, (9 June 2004); https://doi.org/10.1117/12.566616

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