Optical tweezers as a tool for microrheology of simplex and complex fluids

Giuseppe Pesce; Antonio Sasso; Sabatino Fusco; Assunta Borzacchiello; Paolo Netti

doi:10.1117/12.557862

18 October 2004 Optical tweezers as a tool for microrheology of simplex and complex fluids

Giuseppe Pesce, Antonio Sasso, Sabatino Fusco, Assunta Borzacchiello, Paolo Netti

Proceedings Volume 5514, Optical Trapping and Optical Micromanipulation; (2004) https://doi.org/10.1117/12.557862
Event: Optical Science and Technology, the SPIE 49th Annual Meeting, 2004, Denver, Colorado, United States

Abstract

Optical tweezers have become a widely used tool for manipulate microscopic objects. Combined with fast and high sensitive position detection techniques, they are suitable for microrheological measurements of viscous and viscoelastic media. Such measurements require the knowledge of absolute displacements of the trapped particles. Unfortunately position detection device need to be calibrated and several methods have been used and reported in literature. We report an accurate study of the behavior of the conversion factor in the case of the forward light scattering technique, based on an oil-immersion objective lens, which is commonly used in optical tweezers systems. We show how this fundamental parameter is strongly dependent on the distance of the trapped object from the coverslip surface.

Citation Download Citation

Giuseppe Pesce, Antonio Sasso, Sabatino Fusco, Assunta Borzacchiello, and Paolo Netti "Optical tweezers as a tool for microrheology of simplex and complex fluids", Proc. SPIE 5514, Optical Trapping and Optical Micromanipulation, (18 October 2004); https://doi.org/10.1117/12.557862

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available